Aconitine-Induced Increase and Decrease of Acetylcholine Release in the Mouse Phrenic Nerve-Hemidiaphragm Muscle Preparation
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چکیده
منابع مشابه
Tubocurarine and pancuronium inhibit evoked release of acetylcholine from the mouse hemidiaphragm preparation.
Using a sensitive radioactive method that measures selectively the evoked release of acetylcholine, it was demonstrated that, when stimulating at 50 Hz, tubocurarine or pancuronium 2 X 10(-5) mol litre-1 or hexamethonium 10(-3) mol litre-1 significantly decreased the evoked release of acetylcholine in the mouse in vitro phrenic nerve-hemidiaphragm preparation.
متن کاملInteractions between tubocurarine, pancuronium and alcuronium demonstrated in the rat phrenic nerve-hemidiaphragm preparation.
The interactions between tubocurarine, pancuronium and alcuronium on neuromuscular transmission have been studied in an isolated neuromuscular preparation (the rat phrenic nerve-hemidiaphragm). Mixtures of tubocurarine and pancuronium, and tubocurarine and alcuronium were found to have a more potent effect than the additive effects of the individual agents when given alone, that is, mixtures we...
متن کامل5-Hydroxytryptamine causes depression of twitch height in the partially curarized rat phrenic nerve hemidiaphragm preparation.
5-Hydroxytryptamine (5-HT) has significant effects on the skeletal neuromuscular junction. We have evaluated the potential neuromodulatory effects of 5-HT on the rat hemidiaphragm using a non-fatiguing pattern of stimulation (0.3 Hz) in a partially curarized preparation using tubocurarine (EC50 = 560 (range 400-650) nmol litre-1). 5-HT was added to the partially curarized preparation in the fol...
متن کاملReversal of BoNT/A-mediated inhibition of muscle paralysis by 3,4-diaminopyridine and roscovitine in mouse phrenic nerve-hemidiaphragm preparations.
Botulinum neurotoxins (BoNTs) comprise a family of neurotoxic proteins synthesized by anaerobic bacteria of the genus Clostridium. Each neurotoxin consists of two polypeptide chains: a 100kDa heavy chain, responsible for binding and internalization into the nerve terminal of cholinergic motoneurons and a 50kDa light chain that mediates cleavage of specific synaptic proteins in the host nerve te...
متن کاملBotulinum Neurotoxins: Qualitative and Quantitative Analysis Using the Mouse Phrenic Nerve Hemidiaphragm Assay (MPN)
The historical method for the detection of botulinum neurotoxin (BoNT) is represented by the mouse bioassay (MBA) measuring the animal survival rate. Since the endpoint of the MBA is the death of the mice due to paralysis of the respiratory muscle, an ex vivo animal replacement method, called mouse phrenic nerve (MPN) assay, employs the isolated N. phrenicus-hemidiaphragm tissue. Here, BoNT cau...
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ژورنال
عنوان ژورنال: Japanese Journal of Pharmacology
سال: 1994
ISSN: 0021-5198
DOI: 10.1254/jjp.66.421